ABI RPMI 1640-Pen/Strep, HEPES, L-Gln, 10 X 500 mL
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ABI RPMI 1640-Pen/Strep, HEPES, L-Gln, 10 X 500 mL
RPMI 1640 is essential for the culture of mammalian myeloma and hybridoma.
ABI RPMI 1640-Pen/Strep, HEPES, L-Gln, 10 X 500 mL
ABI

Artikelnr.: 5262223

ABI RPMI 1640-Pen/Strep, HEPES, L-Gln, 10 X 500 mL

Artikelnr.: 5262223

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RPMI 1640 is essential for the culture of mammalian myeloma and hybridoma.
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Wat opvalt

Comprehensive Medium
RPMI 1640 with Pen/Strep, HEPES, and L-Gln supports optimal cell culture conditions, ensuring robust growth and maintenance of various cell lines.
Convenient Packaging
Available in 10X concentration and 500 mL bottles, this formulation provides ease of use and storage, minimizing preparation time for researchers.
Enhanced Stability
The inclusion of HEPES buffers helps maintain physiological pH during experiments, ensuring reliable results and improved experimental reproducibility.

Productdetails

Shop for RPMI 1640 Medium with Glutamine, Penicillin, and Streptomycin at Ubuy curacao Global Store. Suitable for the culture of mammalian myeloma, hybridoma, and human leukocytes.
Item Weight1.5 lbs (680 grams)

Voor wie is dit geschikt?

Suitable For
  • Cell Culture Labs

    Ideal for laboratories focused on culturing human and animal cells, providing essential nutrients for various cell lines.

  • Research Institutions

    Suitable for academic and commercial research institutions conducting experiments in cellular biology and related fields.

  • Tissue Engineering

    Beneficial for projects involving tissue engineering, ensuring optimal conditions for cell growth and maintenance.

Not Suitable For
  • Non-Cell Applications

    Not suitable for applications outside of cell culture, such as non-biological research projects or chemical assays.

  • Starter Laboratories

    Beginner labs lacking infrastructure may find it hard to utilize the complexities of RPMI 1640 effectively.

  • Stable Cell Lines

    Not ideal for use with certain stable cell lines requiring specialized media formulations that differ from RPMI 1640.

PRODUCTBESCHRIJVING

ABI RPMI 1640-Pen/Strep, HEPES, L-Gln, 10 X 500 mL

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Vragen & Antwoorden van klanten

  • vraag: What is RPMI 1640-PenStrep, HEPES, and L-Gln used for?

    antwoord: RPMI 1640-PenStrep, HEPES, and L-Gln is primarily used for cell culture, particularly in the growth of lymphocyte and hybridoma cells. This medium provides essential nutrients, vitamins, and salts that enable optimal cell health and proliferation. Additionally, the Penicillin-Streptomycin (PenStrep) helps to prevent bacterial contamination, while HEPES buffers maintain pH stability in varying CO2 conditions. It is widely used in laboratories focused on research, vaccine development, and drug testing.
  • vraag: What types of cells can be cultured in RPMI 1640-PenStrep, HEPES, and L-Gln?

    antwoord: This specific medium is suitable for a variety of cell types, particularly lymphoid cells, such as human T and B lymphocytes, as well as hybridoma cells. It provides the necessary nutrients, growth factors, and buffering capacity for these sensitive and fastidious cells. Researchers commonly use it to study immune responses, cancer biology, and genetic modifications in cell lines, making it a versatile choice in cellular and molecular biology.
  • vraag: How should RPMI 1640-PenStrep, HEPES, and L-Gln be stored?

    antwoord: To maintain the integrity and effectiveness of RPMI 1640-PenStrep, HEPES, and L-Gln, it is recommended to store it in a cool environment, typically at 2-8°C. Avoid repeated freeze-thaw cycles, as they can damage the components of the medium. If opened, it is best to use it within a reasonable time frame to avoid contamination or degradation. Proper storage ensures that your cell cultures receive the highest quality nutrients and supports consistent experimental results.
  • vraag: Can RPMI 1640-PenStrep, HEPES, and L-Gln be supplemented with additional components?

    antwoord: Yes, RPMI 1640-PenStrep, HEPES, and L-Gln can be supplemented with additional components such as serum (like FBS) or specific growth factors to cater to the needs of particular cell types. These supplements can enhance cell growth and viability, leading to optimized conditions for specific experiments. Researchers often customize the medium based on their experimental design, whether it’s for routine cell maintenance, transfection, or differentiation protocols.
  • vraag: Is RPMI 1640-PenStrep, HEPES, and L-Gln suitable for long-term cell culture?

    antwoord: While RPMI 1640-PenStrep, HEPES, and L-Gln is excellent for short to medium-term culture, for long-term cultures, it is advisable to monitor cell viability and replenishment of nutrients regularly. Depending on the specific cell type, researchers may find it beneficial to switch to a medium designed for extended culture periods that includes slower-release nutrients. Monitoring the pH and nutrient levels is essential for maintaining optimal conditions over time.
  • vraag: What are the key benefits of using HEPES in the RPMI 1640 medium?

    antwoord: HEPES is a buffering agent included in RPMI 1640 that helps maintain pH stability in cell culture, particularly in environments that experience fluctuations in CO2 levels. By ensuring a consistent pH, HEPES supports optimal cell growth and function, preventing stress that could alter cell behavior or experiment outcomes. This is especially important for sensitive assays such as drug testing, where even minor pH shifts can significantly impact results.
  • vraag: What is the significance of Penicillin-Streptomycin (PenStrep) in this medium?

    antwoord: PenStrep plays a critical role in RPMI 1640 by preventing bacterial contamination in cell cultures. This antibiotic combination helps to safeguard experimental integrity by inhibiting bacterial growth, which could otherwise compromise cell viability and skew experimental results. It is particularly beneficial in settings where the risk of contamination is higher, such as open laboratory environments or when handling multiple cell lines.
  • vraag: What does the '10X' designation mean in RPMI 1640-PenStrep, HEPES, and L-Gln?

    antwoord: The '10X' designation indicates that the medium is a concentrate, meaning it should be diluted to 1X concentration before use in cell culture. This allows for flexibility in preparation and helps researchers maintain efficiency in their lab practices. By only needing to dilute the medium at the time of use, labs can manage storage and prepare fresh cultures with precise concentrations tailored to specific experimental needs.
  • vraag: Are there any alternatives to RPMI 1640-PenStrep, HEPES, and L-Gln?

    antwoord: Yes, alternatives to RPMI 1640-PenStrep, HEPES, and L-Gln include DMEM, F-10, and MEM, which may be more suitable for certain cells or experimental conditions. Each medium has unique formulations that cater to different cell types and applications. Researchers should choose based on the specific requirements of their cell cultures, such as nutrient needs or environmental sensitivity, to optimize growth and experimental results.
  • vraag: Where can I buy RPMI 1640-PenStrep, HEPES, L-Gln, 10 X 500 mL?

    antwoord: You can purchase RPMI 1640-PenStrep, HEPES, L-Gln, 10 X 500 mL at Ubuy. Ubuy offers a reliable platform to find various laboratory supplies, including cell culture media, allowing you to compare products and select what best meets your needs.

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Voordelen

  • High-quality growth medium
  • Supports various cell types
  • Sterility ensures reliable results
  • Convenient 10X concentration
  • Easy to use and store

Nadelen

  • Might require additional supplements for specific cells.

Prijs-/productgeschiedenis

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ANG 1498

Bestel nu en ontvang het rond Woensdag, Juli 01

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Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.

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Kenmerken en voordelen

  • Developed at Roswell Park Memorial Institute.
  • Ideal for culturing mammalian myeloma and hybridoma cells.
  • Suitable for mouse and human leukocytes, B and T lymphocytes.
  • Available in different formulations tailored to your needs.
  • Includes essential supplements: Glutamine, Penicillin, and Streptomycin.
  • Cell Culture Tested to ensure reliability and sterility.