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Artikelnr.:
139063407
NP40 Cell Lysis Buffer (2X), 7.4 pH, 125mL
Artikelnr.:
139063407
ANG 120
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Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers ensure the highest quality for your research needs.
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Wat opvalt
Effective Cell Lysis
NP40 Cell Lysis Buffer efficiently breaks down cell membranes, ensuring optimal release of cellular contents for accurate analysis, outperforming standard buffers in various applications.
Neutral pH Stability
Formulated at pH 7.4, this buffer maintains biological activity and integrity of sensitive biomolecules, providing reliability and consistency essential for experimental reproducibility.
Convenient Packaging
The 125mL size is ideal for frequent laboratory use, allowing for easy handling and storage without compromising the quality and effectiveness of the lysis process in cell preparation.
Productdetails
- Manufactured in an ISO 9001 and 13485 certified facility in the USA
- For research use only. Not for human or animal clinical/therapeutic use.
- Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests under certified 14644 clean room conditions
- NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting. It can also be used as a wash buffer for immunoprecipitation reactions. It is soluble in water.
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 4 ounces (113.4 grams) |
| Volume | 125 ml |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Included Components | Bottle |
| Part Number | 23.105.113 |
| Brand | eCon-Lab-Supply |
| Item Volume | 125 ml |
| Package Information | Bottle |
Inhoud van de verpakking
Bottle
Voor wie is dit geschikt?
-
Biotechnology Researchers
Ideal for researchers conducting experiments in cell biology requiring cell lysis for protein extraction and analysis.
-
Molecular Biologists
Beneficial for molecular biologists isolating nucleic acids from cell samples through efficient lysis techniques.
-
Academic Laboratories
Perfect for educational settings where practical experimentation with cell lysates is part of the curriculum.
-
Home Users
Not suitable for casual home experiments due to specialized nature and required safety protocols of the buffer.
PRODUCTBESCHRIJVING
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Vragen en antwoorden van klanten
-
vraag:
What is NP40 Cell Lysis Buffer 2X used for?
antwoord: NP40 Cell Lysis Buffer 2X is primarily used to lyse cells, making it an essential reagent in molecular biology and biochemistry. It helps to disrupt cell membranes, allowing researchers to extract proteins, DNA, RNA, and other cellular components. This buffer is particularly useful for studies involving protein expression, purification, and analysis in both clinical and research settings. -
vraag:
How should NP40 Cell Lysis Buffer 2X be prepared for use?
antwoord: The NP40 Cell Lysis Buffer 2X is typically supplied as a concentrated solution. To prepare it for use, simply dilute it with an appropriate buffer or distilled water as recommended in your experimental protocol. This allows for optimal lysis while ensuring compatibility with various downstream applications such as Western blotting, ELISA, or PCR analysis. -
vraag:
Can NP40 Cell Lysis Buffer be used for various cell types?
antwoord: Yes, NP40 Cell Lysis Buffer is versatile and can be used on multiple cell types, including mammalian, bacterial, and yeast cells. However, keep in mind that each cell type may have specific requirements for lysis conditions, such as incubation time and temperature. For best results, refer to the protocols designed for the specific cells you are working with. -
vraag:
Is NP40 Cell Lysis Buffer compatible with downstream applications?
antwoord: Absolutely, NP40 Cell Lysis Buffer is designed to be compatible with a wide range of downstream applications such as protein analysis, immunoprecipitation, and nucleic acid extraction. It provides a balance of cell disruption while preserving the integrity of biomolecules, making it ideal for various experimental protocols. -
vraag:
What is the pH of NP40 Cell Lysis Buffer 2X, and why is it important?
antwoord: The NP40 Cell Lysis Buffer comes at a pH of 7.4, which is critical for maintaining the stability of biomolecules during the lysis process. This neutral pH promotes optimal enzyme activity and prevents denaturation of proteins, ensuring that the extracted components are functional and suitable for analysis. -
vraag:
How long can I store NP40 Cell Lysis Buffer 2X?
antwoord: NP40 Cell Lysis Buffer 2X can generally be stored at 4°C for short-term use, but for long-term storage, it is often recommended to keep it at -20°C. Proper storage conditions help maintain the buffer's efficacy and stability, allowing for reliable results in your experiments over time. -
vraag:
Is NP40 Cell Lysis Buffer 2X toxic or hazardous?
antwoord: NP40 Cell Lysis Buffer contains detergents that can be hazardous if not handled properly. Therefore, it is important to use personal protective equipment, such as gloves and goggles, while working with the buffer. Adhering to safety protocols can help ensure a safe working environment during your experiments. -
vraag:
Can I use NP40 Cell Lysis Buffer 2X for both protein and nucleic acid extraction?
antwoord: Yes, NP40 Cell Lysis Buffer 2X is suitable for both protein and nucleic acid extraction. Its formulation allows for efficient cell lysis while preserving the integrity of proteins and nucleic acids, which can be particularly beneficial in experiments where both types of biomolecules are required for analysis or further study. -
vraag:
What cell lysis protocols can be used with NP40 Cell Lysis Buffer?
antwoord: Protocols for cell lysis with NP40 Cell Lysis Buffer can vary depending on the type of cells being lysed. Common approaches include sonication, freeze-thaw cycles, or enzymatic digestion to enhance lysis efficiency. It’s essential to refer to specific guidelines based on your experimental needs to achieve optimal results. -
vraag:
Where can I buy NP40 Cell Lysis Buffer 2X, 7.4 pH, 125mL in curacao?
antwoord: You can purchase NP40 Cell Lysis Buffer 2X, 7.4 pH, 125mL from Ubuy, which offers a reliable source for various laboratory reagents and products. Ubuy provides a convenient platform to find this essential buffer along with other scientific supplies tailored to your needs.
eCon Lab Supply Buffers Editorial Review
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Kenmerken en voordelen
- Comprehensive range of biological buffers for various applications.
- Ideal for protein research, molecular biology, and characterization.
- Produced in a certified facility adhering to stringent quality control.
- NP40 Cell Lysis Buffer is perfect for Antibody Bead Immunoassay and ELISA.
- Designed for cell extract preparation and immunoprecipitation wash.
- Soluble in water for easy use in laboratory settings.
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