Tris.HCl (AR Grade, 500g)
ANG 136
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Experience the purity with our ULTRA PURE Tris HCl, boasting an assay of ≥99.5%!
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Wat opvalt
Productdetails
| Item Weight | 1.1 lbs (500 grams) |
Voor wie is dit geschikt?
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Laboratory Researchers
Ideal for researchers conducting biological studies requiring precise pH buffering and stable conditions for experiments.
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Educational Institutions
Perfect for universities and schools that need high-quality chemicals for chemistry and biology lab classes.
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Biotechnology Firms
Beneficial for biotech companies performing molecular biology protocols, especially in DNA and RNA analyses.
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General Consumers
Not suitable for non-professionals or general consumers who lack experience or knowledge in chemical usage.
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Non-Academic Uses
Not recommended for applications outside of laboratory settings, such as home or industrial use without guidance.
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Casual Hobbyists
Hobbyists may find it unnecessary; specialized knowledge is essential for safe and effective handling of such chemicals.
PRODUCTBESCHRIJVING
Tris.HCl (AR Grade, 500g)
Vragen & Antwoorden van klanten
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vraag:
What is Tris.HCl and what is its primary use?
antwoord: Tris.HCl, also known as Tris buffer, is a widely used buffering agent in biological and biochemical research. Its primary use is to maintain a stable pH in various laboratory applications, including molecular biology, biochemistry, and analytical chemistry. This stability is essential during reactions, allowing for accurate results when working with proteins, nucleic acids, and other sensitive biological materials. -
vraag:
How should Tris.HCl be stored for optimal quality?
antwoord: Tris.HCl should be stored in a cool, dry place, away from direct sunlight and moisture. Upon opening, it is recommended to keep it in a tightly sealed container to prevent contamination and degradation. Proper storage ensures that the buffer maintains its chemical integrity, thus providing reliable performance in various experimental protocols. -
vraag:
Can I use Tris.HCl for preparing DNA and RNA solutions?
antwoord: Yes, Tris.HCl is commonly used for preparing solutions for DNA and RNA work. It helps in maintaining the pH stability which is crucial for the integrity of nucleic acids during handling and storage. Researchers rely on this buffer when performing techniques such as electrophoresis and PCR to ensure optimal conditions for the nucleic acids. -
vraag:
What are the typical applications of Tris.HCl in the laboratory?
antwoord: Tris.HCl is extensively utilized in molecular biology applications, including protein electrophoresis, enzyme assays, and DNA amplification techniques. Additionally, it's used to formulate buffers for cell culture and various biochemical assays, making it an essential reagent for laboratories across many scientific disciplines. -
vraag:
Is Tris.HCl suitable for sensitive assays?
antwoord: Absolutely, Tris.HCl is often ideal for sensitive assays due to its ability to maintain a consistent pH, which is crucial for many biological reactions. This characteristic makes it a favorite in high-precision experiments, such as enzyme kinetics and protein assays, where even slight pH variations can significantly impact results. -
vraag:
Is Tris.HCl safe to handle in a laboratory environment?
antwoord: Tris.HCl is generally considered to be safe to handle, but as with all chemicals, it requires proper handling procedures. Wearing gloves, goggles, and a lab coat is advisable to minimize exposure. Understanding safety data sheets (SDS) ensures that users are aware of any potential hazards and can conduct their work safely. -
vraag:
What pH range is typically maintained using Tris.HCl?
antwoord: Tris.HCl is capable of buffering solutions in a pH range of approximately 7 to 9. This makes it suitable for a variety of biological applications, as many enzymatic reactions and cellular processes operate optimally within this pH range. Researchers can prepare specific Tris buffers adjusted to the desired pH for their experiments. -
vraag:
How do I prepare a Tris buffer solution?
antwoord: To prepare a Tris buffer solution, dissolve the appropriate amount of Tris.HCl powder in distilled water, then adjust the pH using HCl or NaOH as needed. The concentration can vary depending on the application, but a standard preparation might be 0.1M Tris at a pH of 7.5. This method ensures that the buffer is tailored to specific experimental requirements. -
vraag:
Can Tris.HCl be used in cell culture media?
antwoord: Yes, Tris.HCl is often included in cell culture media as a buffering agent. It helps to maintain a stable pH, which is crucial for cell growth and function. Researchers frequently add Tris to their formulations to ensure optimal cellular environments, especially when working with sensitive cell lines or primary cultures. -
vraag:
Where can I buy Tris.HCl AR Grade, 500g in curacao?
antwoord: You can purchase Tris.HCl AR Grade, 500g from Ubuy in curacao. Ubuy offers a variety of laboratory chemicals and ensures that you receive high-quality products suitable for your scientific and research needs.
Analytical Reagents Editorial Review
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Voordelen
- High purity for reliable results
- Easily soluble in water
- Suitable for various applications
- Stable and long shelf-life
- Well-packaged for laboratory use
Nadelen
- Occasional settling in storage
Prijs-/productgeschiedenis
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ANG 136
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Kenmerken en voordelen
- ULTRA PURE Tris HCl with an assay of ≥99.5%
- CAS Number: 1185-53-1 for reliable identification
- Molecular Weight: 157.64 ensures consistent quality
- White crystalline appearance signifies high quality
- Store at room temperature for optimal longevity
- Perfect for various laboratory applications